Jenway 6850 User Manual

Operating Manual 685 030 REV B/06-14 6850 Spectrophotometer

10 The instrument will initially perform several power-on tests before displaying the warm up screen: Fig 2.2 – Instrument power-on tests complet

11 2.4 CONTROLS The keypad used for this model enables an easy and effective way of navigating the different measurement modes, entering number

12 ESC Key: Return to previous menu screen 100%T/0Abs Key: Zero / Blank instrument OPEN Key: Open results stored in internal memory SAVE Key: S

13 2.5 REAR PANEL The image below shows the rear panel of the instrument: Fig. 2.5 – Rear Panel 1. Fan Cover Allows access to lamp when

14 SECTION 3 – THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS 3.1 THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement

15 3.2 NUCLEIC ACID DETERMINATION DNA, RNA and oligonucleotides can be measured directly in aqueous solutions in a diluted or undilu

16 3.4 GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in

17 h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and was

18 SECTION 4 – INSTRUMENT SETUP 4.1 INSTRUMENT NAVIGATION To navigate around the spectrophotometer screen press the arrow keys on the main keypad

19 4.3 SYSTEM UTILITY MENU System Utility Menu The system utility menu option allows the user to perform a variety of setup and service f

2 Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed to b

20 4.3.3 Lamp Setup In the lamp setup menu the user can perform the following tasks. 1. Switch on/off the deuterium lamp 2. Reset the

21 4.3.4.2 Set Date Highlight the set date option and press the Enter key to confirm. This will allow the user to enter the current date in the

22 4.3.10 Delete Entire Saved Files Highlight the delete entire saved files option and press the Enter key to confirm. A warning will be shown on

23 SECTION 5 – PHOTOMETRICS The photometrics measurement mode enables simple measurements of absorbance, % transmittance and concentration

24 5.2.3 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option. The require

25 5.3.2 Calibrating to a Standard A calibration standard can be used to determine the concentration factor. Insert the cuvette cont

26 5.4.3 Measuring a Sample after Calibrating to a Standard Remove the cuvette containing the standard sample and place a cuvette conta

27 SECTION 6 – QUANTITATION The quantitation measurement mode enables sample concentrations to be calculated using a standard curve. I

28 6.3.1 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option. The requir

29 6.3.2.2 Manually Enter Curve Constants The user can manually enter the calibration curve constants by pressing the function k

3 Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch. 1. Das in diesem Handbuch besch

30 6.4 CREATING A NEW STANDARD CURVE The user must firstly edit the calibration table before attempting to create a new standard curve. Insert

31 6.5 STORING A STANDARD CURVE A standard curve can be saved to the instrument’s internal memory to allow it to be recalled for future

32 6.7.1 Quantitation Measurements Ensure that a cuvette containing the blank solution is in the reference position in the sample chamber

33 SECTION 7 – SPECTRUM The spectrum measurement mode enables measurements of absorbance or % transmittance over a range of wavelengths to be perfo

34 7.3 SELECTING THE MEASUREMENT MODE The measurement mode can be selected by pressing the function key below the Mode option. The required measur

35 7.6 SPECTRUM SEARCH The photometric values of the measured spectrum can be viewed by pressing the function key below the Search optio

36 7.9 RECALLING STORED RESULTS A result file can be opened from the instrument’s internal memory. Press the Open key on the instru

37 SECTION 8 – KINETICS The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be measured over a per

38 8.3.1 Selecting the Measurement Mode The measurement mode can be selected by pressing the function key below the Mode option. The required meas

39 8.6 CALCULATING I/U CONCENTRATION The instrument software allows the user to calculate the concentration of a substance from th

4 4. È sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche utilizzate. Adotta

40 8.8 STORING RESULTS A result file can be saved to the instrument’s internal memory. Press the Save key on the instrument keypa

41 SECTION 9 – DNA/PROTEIN The DNA/Protein measurement mode allows the user to measure multi-wavelength absorbance ratios, such as 260nm/

42 Absorbance Difference 2 Purity Ratio = Abs@260nm / Abs@230nm RNA Conc (μg/ml) = (Abs@260 x 49.1) – (Abs@230 x 3.48) Protein Conc (μ

43 9.6 RESET MODE SETTINGS The DNA/Protein mode settings can be reset to their default values by pressing the function key below th

44 9.9 RECALLING STORED RESULTS A method/result file can be opened from the instrument’s internal memory. In the DNA/Protein mode screen

45 SECTION 10 – MULTI-WAVELENGTH The multi-wavelength measurement mode allows the user to measure a sample’s photometric absorbance or

46 10.3 SAMPLE MEASUREMENT Insert a cuvette containing the sample to be analysed into the sample chamber and press the key below the

47 SECTION 11 – SAVING/RECALLING RESULTS USING A USB MEMORY STICK There is a USB port on the side of the instrument for data storage. Please no

48 SECTION 12 – ACCESSORIES AND SPARE PARTS 11.1 OPTIONAL ACCESSORIES Part Code Description of Accessory 685 204 10 x 10mm path length cuvette hol

49 11.2.2 Active Accessories 11.2.2.1 8 Position Automatic Cell Changer Unscrew the two fixing screws to undo the passive accessory. L

5 Contents SECTION 1 – Introduction ... 8 1.1 INSTRUMENT DE

50 Place the accessory in the correct orientation and re-tighten the two fixing screws to fix the 8 cell accessory in place. 11.3 USING

51 11.4 SPARES Part Code Description of Spare Part 685 020 Tungsten lamp 685 021 Deuterium lamp

52 SECTION 12 – MAINTENANCE AND SETVICE 12.1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The samp

53 3. Whilst wearing cotton gloves remove the defective tungsten lamp and replace with a new one. 4. Switch on the power and m

54 12.3 SERVICE Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment develops a fault. Please contact t

55 SECTION 13 – TROUBLESHOOTING 13.1 TROUBLESHOOTING GUIDE Issue Solution Unable to achieve a reading when measuring a sample Ensure the corr

56 SECTION 14 – DECLARATION OF CONFORMITY

6 5.4 SAMPLE MEASUREMENT ... 25 5.4.1 Photometric Me

7 9.8 STORING METHODS/RESULTS ... 43 9.9 RECALLING STORED RES

8 SECTION 1 – Introduction 1.1 INSTRUMENT DESCRIPTION The 6850 is a variable bandwidth, double beam UV/visible spectrophotometer with an integrate

9 Protein Measurement modes Direct UV Other Beam height 15mm Light source Tungsten and Deuterium lamps Outputs USB, Parallel (Printer) Power 110/22